An AGAMOUS intron-driven cytotoxin leads to flowerless tobacco and produces no detrimental effects on vegetative growth of either tobacco or poplar.
Identifieur interne : 001A12 ( Main/Exploration ); précédent : 001A11; suivant : 001A13An AGAMOUS intron-driven cytotoxin leads to flowerless tobacco and produces no detrimental effects on vegetative growth of either tobacco or poplar.
Auteurs : Wei Li [États-Unis] ; Wei Hu [États-Unis] ; Chu Fang [États-Unis] ; Longzheng Chen [États-Unis, République populaire de Chine] ; Weibing Zhuang [États-Unis, République populaire de Chine] ; Lorenzo Katin-Grazzini [États-Unis] ; Richard J. Mcavoy [États-Unis] ; Karl Guillard [États-Unis] ; Yi Li [États-Unis, République populaire de Chine]Source :
- Plant biotechnology journal [ 1467-7652 ] ; 2016.
Descripteurs français
- KwdFr :
- Fleurs (génétique), Fleurs (métabolisme), Introns (génétique), Populus (génétique), Populus (métabolisme), Protéines végétales (génétique), Protéines végétales (métabolisme), Régions promotrices (génétique) (génétique), Régulation de l'expression des gènes végétaux (MeSH), Tabac (génétique), Tabac (métabolisme).
- MESH :
- génétique : Fleurs, Introns, Populus, Protéines végétales, Régions promotrices (génétique), Tabac.
- métabolisme : Fleurs, Populus, Protéines végétales, Tabac.
- Régulation de l'expression des gènes végétaux.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Plant Proteins.
- genetics : Flowers, Introns, Populus, Promoter Regions, Genetic, Tobacco.
- metabolism : Flowers, Plant Proteins, Populus, Tobacco.
- Gene Expression Regulation, Plant.
Abstract
Flowerless trait is highly desirable for poplar because it can prevent pollen- and seed-mediated transgene flow. We have isolated the second intron of PTAG2, an AGAMOUS (AG) orthologue from Populus trichocarpa. By fusing this intron sequence to a minimal 35S promoter sequence, we created two artificial promoters, fPTAG2I (forward orientation of the PTAG2 intron sequence) and rPTAG2I (reverse orientation of the PTAG2 intron sequence). In tobacco, expression of the β-glucuronidase gene (uidA) demonstrates that the fPTAG2I promoter is non-floral-specific, while the rPTAG2I promoter is active in floral buds but with no detectable vegetative activity. Under glasshouse conditions, transgenic tobacco plants expressing the Diphtheria toxin A (DT-A) gene driven by the rPTAG2I promoter produced three floral ablation phenotypes: flowerless, neuter (stamenless and carpel-less) and carpel-less. Further, the vegetative growth of these transgenic lines was similar to that of the wild-type plants. In field trials during 2014 and 2015, the flowerless transgenic tobacco stably maintained its flowerless phenotype, and also produced more shoot and root biomass when compared to wild-type plants. In poplar, the rPTAG2I::GUS gene exhibited no detectable activity in vegetative organs. Under field conditions over two growing seasons (2014 to the end of 2015), vegetative growth of the rPTAG2I::DT-A transgenic poplar plants was similar to that of the wild-type plants. Our results demonstrate that the rPTAG2I artificial promoter has no detectable activities in vegetative tissues and organs, and the rPTAG2I::DT-A gene may be useful for producing flowerless poplar that retains normal vegetative growth.
DOI: 10.1111/pbi.12581
PubMed: 27168170
PubMed Central: PMC5103258
Affiliations:
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Le document en format XML
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Li</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT</wicri:regionArea><placeName><region type="state">Connecticut</region></placeName></affiliation></author><author><name sortKey="Hu, Wei" sort="Hu, Wei" uniqKey="Hu W" first="Wei" last="Hu">Wei Hu</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT</wicri:regionArea><placeName><region type="state">Connecticut</region></placeName></affiliation></author><author><name sortKey="Fang, Chu" sort="Fang, Chu" uniqKey="Fang C" first="Chu" last="Fang">Chu Fang</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT</wicri:regionArea><placeName><region type="state">Connecticut</region></placeName></affiliation></author><author><name sortKey="Chen, Longzheng" sort="Chen, Longzheng" uniqKey="Chen L" first="Longzheng" last="Chen">Longzheng Chen</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT</wicri:regionArea><placeName><region type="state">Connecticut</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Zhuang, Weibing" sort="Zhuang, Weibing" uniqKey="Zhuang W" first="Weibing" last="Zhuang">Weibing Zhuang</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT</wicri:regionArea><placeName><region type="state">Connecticut</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>College of Horticulture and State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Horticulture and State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Katin Grazzini, Lorenzo" sort="Katin Grazzini, Lorenzo" uniqKey="Katin Grazzini L" first="Lorenzo" last="Katin-Grazzini">Lorenzo Katin-Grazzini</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT</wicri:regionArea><placeName><region type="state">Connecticut</region></placeName></affiliation></author><author><name sortKey="Mcavoy, Richard J" sort="Mcavoy, Richard J" uniqKey="Mcavoy R" first="Richard J" last="Mcavoy">Richard J. Mcavoy</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT</wicri:regionArea><placeName><region type="state">Connecticut</region></placeName></affiliation></author><author><name sortKey="Guillard, Karl" sort="Guillard, Karl" uniqKey="Guillard K" first="Karl" last="Guillard">Karl Guillard</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT</wicri:regionArea><placeName><region type="state">Connecticut</region></placeName></affiliation></author><author><name sortKey="Li, Yi" sort="Li, Yi" uniqKey="Li Y" first="Yi" last="Li">Yi Li</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT</wicri:regionArea><placeName><region type="state">Connecticut</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>College of Horticulture and State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Horticulture and State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author></analytic><series><title level="j">Plant biotechnology journal</title><idno type="eISSN">1467-7652</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Flowers (genetics)</term><term>Flowers (metabolism)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Introns (genetics)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term><term>Promoter Regions, Genetic (genetics)</term><term>Tobacco (genetics)</term><term>Tobacco (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Fleurs (génétique)</term><term>Fleurs (métabolisme)</term><term>Introns (génétique)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Régions promotrices (génétique) (génétique)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Tabac (génétique)</term><term>Tabac (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Flowers</term><term>Introns</term><term>Populus</term><term>Promoter Regions, Genetic</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Fleurs</term><term>Introns</term><term>Populus</term><term>Protéines végétales</term><term>Régions promotrices (génétique)</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Flowers</term><term>Plant Proteins</term><term>Populus</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Fleurs</term><term>Populus</term><term>Protéines végétales</term><term>Tabac</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Régulation de l'expression des gènes végétaux</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Flowerless trait is highly desirable for poplar because it can prevent pollen- and seed-mediated transgene flow. We have isolated the second intron of PTAG2, an AGAMOUS (AG) orthologue from Populus trichocarpa. By fusing this intron sequence to a minimal 35S promoter sequence, we created two artificial promoters, fPTAG2I (forward orientation of the PTAG2 intron sequence) and rPTAG2I (reverse orientation of the PTAG2 intron sequence). In tobacco, expression of the β-glucuronidase gene (uidA) demonstrates that the fPTAG2I promoter is non-floral-specific, while the rPTAG2I promoter is active in floral buds but with no detectable vegetative activity. Under glasshouse conditions, transgenic tobacco plants expressing the Diphtheria toxin A (DT-A) gene driven by the rPTAG2I promoter produced three floral ablation phenotypes: flowerless, neuter (stamenless and carpel-less) and carpel-less. Further, the vegetative growth of these transgenic lines was similar to that of the wild-type plants. In field trials during 2014 and 2015, the flowerless transgenic tobacco stably maintained its flowerless phenotype, and also produced more shoot and root biomass when compared to wild-type plants. In poplar, the rPTAG2I::GUS gene exhibited no detectable activity in vegetative organs. Under field conditions over two growing seasons (2014 to the end of 2015), vegetative growth of the rPTAG2I::DT-A transgenic poplar plants was similar to that of the wild-type plants. Our results demonstrate that the rPTAG2I artificial promoter has no detectable activities in vegetative tissues and organs, and the rPTAG2I::DT-A gene may be useful for producing flowerless poplar that retains normal vegetative growth.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27168170</PMID><DateCompleted><Year>2017</Year><Month>09</Month><Day>01</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1467-7652</ISSN><JournalIssue CitedMedium="Internet"><Volume>14</Volume><Issue>12</Issue><PubDate><Year>2016</Year><Month>12</Month></PubDate></JournalIssue><Title>Plant biotechnology journal</Title><ISOAbbreviation>Plant Biotechnol J</ISOAbbreviation></Journal><ArticleTitle>An AGAMOUS intron-driven cytotoxin leads to flowerless tobacco and produces no detrimental effects on vegetative growth of either tobacco or poplar.</ArticleTitle><Pagination><MedlinePgn>2276-2287</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/pbi.12581</ELocationID><Abstract><AbstractText>Flowerless trait is highly desirable for poplar because it can prevent pollen- and seed-mediated transgene flow. We have isolated the second intron of PTAG2, an AGAMOUS (AG) orthologue from Populus trichocarpa. By fusing this intron sequence to a minimal 35S promoter sequence, we created two artificial promoters, fPTAG2I (forward orientation of the PTAG2 intron sequence) and rPTAG2I (reverse orientation of the PTAG2 intron sequence). In tobacco, expression of the β-glucuronidase gene (uidA) demonstrates that the fPTAG2I promoter is non-floral-specific, while the rPTAG2I promoter is active in floral buds but with no detectable vegetative activity. Under glasshouse conditions, transgenic tobacco plants expressing the Diphtheria toxin A (DT-A) gene driven by the rPTAG2I promoter produced three floral ablation phenotypes: flowerless, neuter (stamenless and carpel-less) and carpel-less. Further, the vegetative growth of these transgenic lines was similar to that of the wild-type plants. In field trials during 2014 and 2015, the flowerless transgenic tobacco stably maintained its flowerless phenotype, and also produced more shoot and root biomass when compared to wild-type plants. In poplar, the rPTAG2I::GUS gene exhibited no detectable activity in vegetative organs. Under field conditions over two growing seasons (2014 to the end of 2015), vegetative growth of the rPTAG2I::DT-A transgenic poplar plants was similar to that of the wild-type plants. Our results demonstrate that the rPTAG2I artificial promoter has no detectable activities in vegetative tissues and organs, and the rPTAG2I::DT-A gene may be useful for producing flowerless poplar that retains normal vegetative growth.</AbstractText><CopyrightInformation>© 2016 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Wei</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Wei</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fang</LastName><ForeName>Chu</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Longzheng</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhuang</LastName><ForeName>Weibing</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Horticulture and State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Katin-Grazzini</LastName><ForeName>Lorenzo</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>McAvoy</LastName><ForeName>Richard J</ForeName><Initials>RJ</Initials><AffiliationInfo><Affiliation>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guillard</LastName><ForeName>Karl</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Yi</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Horticulture and State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>AF052571</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>06</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Plant Biotechnol J</MedlineTA><NlmUniqueID>101201889</NlmUniqueID><ISSNLinking>1467-7644</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D035264" MajorTopicYN="N">Flowers</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007438" MajorTopicYN="N">Introns</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">
AGAMOUS
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Hu</name><name sortKey="Katin Grazzini, Lorenzo" sort="Katin Grazzini, Lorenzo" uniqKey="Katin Grazzini L" first="Lorenzo" last="Katin-Grazzini">Lorenzo Katin-Grazzini</name><name sortKey="Li, Yi" sort="Li, Yi" uniqKey="Li Y" first="Yi" last="Li">Yi Li</name><name sortKey="Mcavoy, Richard J" sort="Mcavoy, Richard J" uniqKey="Mcavoy R" first="Richard J" last="Mcavoy">Richard J. Mcavoy</name><name sortKey="Zhuang, Weibing" sort="Zhuang, Weibing" uniqKey="Zhuang W" first="Weibing" last="Zhuang">Weibing Zhuang</name></country><country name="République populaire de Chine"><noRegion><name sortKey="Chen, Longzheng" sort="Chen, Longzheng" uniqKey="Chen L" first="Longzheng" last="Chen">Longzheng Chen</name></noRegion><name sortKey="Li, Yi" sort="Li, Yi" uniqKey="Li Y" first="Yi" last="Li">Yi Li</name><name sortKey="Zhuang, Weibing" sort="Zhuang, Weibing" uniqKey="Zhuang W" first="Weibing" last="Zhuang">Weibing Zhuang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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